Part:BBa_K3166058
IAY(rbs-idi-rbs-ispA-rbs-YSS)
The IAY (rbs-idi-rbs-ispA-rbs-YSS) composed by three genes idi、ispA and YSS as well as relevant rbs is our favorite composite part. IAY can combine with optimized MEP and MYA pathway in various chassis cells, raises the yield of squalene greatly. In E.coli BL21 (DE3) containing p35151, the production of squalene is 3.7 times higher when iay was introduced than that of YSS only; when IAY is added with gene IspH, the production of squalene is 472.3mg/l, 25 times higher when it is combined with the optimized MEP pathway vector pmep-dg; when it is combined with the optimized MVA pathway vectors pmva1 and pmva2, the production of squalene is 974.3mg/l, compared with the strains containing pmva1, pmva2 and YSS, the yield of the strain is increased by 1.6 times, compared with that of the strain containing p35151 and 52 times with YSS.
After replacing the chassis cell with XL1-blue, iay is combined with pmva1 and pmva2, and the yield of squalene is 1.3g/l, 1.3 times higher than that of BL21
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 376
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1422
Illegal SpeI site found at 376 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 376
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 376
Illegal AgeI site found at 718 - 1000COMPATIBLE WITH RFC[1000]
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